Coding

Part:BBa_K1442100

Designed by: Waqar Yousaf   Group: iGEM14_Warwick   (2014-10-06)
Revision as of 15:34, 6 October 2014 by Registry (Talk | contribs)

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RdRP

This is RdRP, or RNA dependent RNA polymerase. That is, it produces a protein which can bind to a single-strand RNA fragment via an IRES (internal ribosome entry site), read the RNA, and produce from it the same RNA fragment in the opposite sense. This was fundamental to our 'Replicon' project, because it allows the RNA (introduced via viral chassis in the theoretical or transformation in the practical) to self-replicate within a cell.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 492
    Illegal BglII site found at 1340
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 537


[edit]
Categories
Parameters
n/aRdRP