Coding
comDE

Part:BBa_K1351016

Designed by: Judith Heckmann   Group: iGEM14_LMU-Munich   (2014-10-02)
Revision as of 15:08, 6 October 2014 by JudithHeckmann (Talk | contribs)

comDE Quorum Sensing two component system (Freiburg Standard)

Quorum sensing two component system (histidine kinase comD, response regulator comE)of Streptococcus pneumoniae (R6) able to sense the competence stimulating peptide CSP. Part contains optimized RBS and Terminator for Bacillus subtilis.

fwd primer:gatcgaattccgcggccgcttctagataaggaggagccggcATGGATTTATTTGGATTTGGGACGG rev primer:gatcctgcagcggccgctactagtattaaccggtCTTTTGAGATTTTTTCTCTAAAATATC

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1164
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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