Coding

Part:BBa_K1403019:Design

Designed by: Paris Bettencourt 2014   Group: iGEM14_aris_Bettencourt   (2014-10-06)
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Acetohydroxybutanoate synthase /acetolactate synthase (ilvB/N) expression cassette


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 47
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 47
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 47
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This sequence was submitted downstream a standard T7 constitutive promoter inside a pETDuet vector.


Source

E. coli MG1655

References