Coding
Intein

Part:BBa_K1483003

Designed by: Nikolas Layer, Philipp H. O. Mayer and Philip Roessler   Group: iGEM14_Tuebingen   (2014-10-06)
Revision as of 13:02, 6 October 2014 by Registry (Talk | contribs)

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Ssp GyrB Split Intein

N-Intein part of the ssp GyrB split intein. Can be used in conjunction with the 6 amino acid long C-Intein to N-terminally immobilize a peptide onto a matrix. For immobilisation, parts need to be fused to the C-terminus of the N-intein. The N-terminus of the C-Intein is fused the matrix. The split intein recombines and splices itself out, thereby specificially immobilising the N-terminus of the peptide to the matrix.

The sequence of the corresponding C-Intein is: H2N-GVPVHN-COOH (was aquired by peptide synthesis and is thus not part of the registry)

This part can also be used in order to create fusion proteins, by attaching the C-Intein to a proein instead of the matrix. Part in RFC25 standard.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None