Device
Part:BBa_K1350006:Experience
Designed by: Yiting Luo Group: iGEM14_SZU-China (2014-09-28)
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K1350006
User Reviews
UNIQ75286e9d15233a10-partinfo-00000000-QINU UNIQ75286e9d15233a10-partinfo-00000001-QINU
Characterization experiment on BBa_K1350006--Mdfa
Mdfa is an antiporter we extracted from E-coli.It enables the E-coli survivie in high pH which can up to pH10. We performed some experiments to prove that the Mdfa really founctions. We performed several experiments with different LB medium. We used: 3o with mdfa in LB pH 7.0 - 8.0 - 9.0 - 9.25 - 9.5 - 9.75 -10 - 10.5 3o without mdfa in LB pH 7.0 - 8.0 - 9.0 - 9.25 - 9.5 - 9.75 -10 - 10.5