Generator

Part:BBa_K1403006:Design

Designed by: Antonio Villarreal Larrauri   Group: iGEM14_aris_Bettencourt   (2014-10-06)
Revision as of 11:31, 6 October 2014 by Registry (Talk | contribs)

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Jasmonic acid carboxyl methyltransferase (JMT) expression cassette


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 222
    Illegal BamHI site found at 793
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 71
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Silently removed an illegal PstI site from the codon optimised sequence. Added a C-terminus His tag.


Source

CDS of JMT (AY008434.1), codon optimised for E. coli.

References