Part:BBa_K1403002

AgaA expression (3M2H-> 3H3MH)

agaA is an enzyme present in Corynebacterium striatum that is the main responsible for body odor. It metabolizes 3-methyl-2-hexenoic acid into 3-hydroxy-3-methylhexanoic acid, that is a sulphure volatile compound.

Name: BBa_K1403002 Description: Biobrick submission of agaA expression construct Resistances : Cm Backbone : pSB1C3 Parts: Pcons - RBS - agaA - his tag Design date: 11/June/2014

This part contains:

1) Plasmid pSB1C3: High copy BioBrick assembly plasmid. Constitutive expression. To use in E coli

2) agaA construct: - Promoter BBa_J23108 from the Anderson's promoter collection

- RBS from the RBS Calculator of Salis lab specific for E coli

- BioBrick Prefix + Scar from the iGEM Parts webpage

- agaA sequence codon optimized for E coli 12 (IDT online tool). We checked with Geneious that there were no restriction sites for EcoRI, SpeI, XbaI and PstI.

- Histidine tag: 6 Histidines in C-terminal were added -Stop codon -BioBrick scar + BioBrick suffix from the iGEM Parts webpage

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 288
  Illegal BamHI site found at 519
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 181
  
• 1000
  COMPATIBLE WITH RFC[1000]