Translational_Unit

Part:BBa_K1465104:Design

Designed by: iGEM-Team Bielefeld 2014   Group: iGEM14_Bielefeld-CeBiTec   (2014-10-05)
Revision as of 22:29, 5 October 2014 by Tore (Talk | contribs) (→‎Source)


Fumarate reductase FumBCD from Actinobacillus succinogenes


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 730
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1069
    Illegal AgeI site found at 1230
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1146


Design Notes

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Source

Gensynthesis ([http://eu.idtdna.com/pages/products/genes/gblocks-gene-fragments gBlocks]) of the codon optimized sequence for Escherchia coli by using the [http://www.jcat.de/ Codon Adaptation Tool] and the [http://eu.idtdna.com/CodonOpt Codon Optimization from IDT]:


BILD

References