Other
Part:BBa_K1402016
Designed by: Chen Xi Group: iGEM14_NJU-QIBEBT (2014-10-04)
Ara-RBS-BTE-Treminator
This part contains two protein binding sites. The first binds the CAP protein, which is generally present in E.coli and is asocciated with cell health and availability of glucose. The second binds AraC protein.In the absence of AraC protein and CAP protein, this part promotes transcription.In the presence of AraC protein and CAP protein, this part inhibits transcription.This is a direct copy of Pbad promoter in E.coli DH5α ,the natural promoter for the Ara operon.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 797
Illegal BglII site found at 937
Illegal BamHI site found at 236
Illegal BamHI site found at 386 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 71
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1346
Illegal SapI site found at 53
[edit]
Categories
Parameters
None |