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Part:BBa_K1402016

Designed by: Chen Xi   Group: iGEM14_NJU-QIBEBT   (2014-10-04)
Revision as of 13:34, 4 October 2014 by Registry (Talk | contribs)

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Ara-RBS-BTE-Treminator

This part contains two protein binding sites. The first binds the CAP protein, which is generally present in E.coli and is asocciated with cell health and availability of glucose. The second binds AraC protein.In the absence of AraC protein and CAP protein, this part promotes transcription.In the presence of AraC protein and CAP protein, this part inhibits transcription.This is a direct copy of Pbad promoter in E.coli DH5α ,the natural promoter for the Ara operon.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 797
    Illegal BglII site found at 937
    Illegal BamHI site found at 236
    Illegal BamHI site found at 386
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 71
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1346
    Illegal SapI site found at 53


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Categories
Parameters
None