Project

Part:BBa_J24666

Designed by: Jamie Lemon   Group: iGEM06_Brown   (2006-06-30)
Revision as of 00:06, 27 September 2006 by Johncumbers (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Toggle Switch to Immobilize E. Coli

This part uses a pLac promoter to create motB and also uses an inverter (cI and pcI) to produce LacI. In addition LasI is produced in the motile state, which creates the signalling molecule AI-1. LasR is a transcription factor created in the immobile state. AI-1 and LasR form a complex to initiate transcription from pLasR promoter. We call this the Freeze Machine.

BrownFreezeMachine.jpg

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 886
    Illegal BglII site found at 4006
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4361
    Illegal AgeI site found at 448
    Illegal AgeI site found at 4558
    Illegal AgeI site found at 4945
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1220


[edit]
Categories
Parameters
None