Device

Part:BBa_K759001:Experience

Designed by: Ichiro Takeuchi   Group: iGEM12_HokkaidoU_Japan   (2012-09-19)
Revision as of 22:25, 3 October 2014 by Anacujba (Talk | contribs) (→‎User Reviews)


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Applications of BBa_K759001

User Reviews

UNIQe60fe25c62557c1e-partinfo-00000000-QINU UNIQe60fe25c62557c1e-partinfo-00000001-QINU • Aim – to verify Assembly Standard 10 compliance
• Methods:

 Standard restriction digest and gel electrophoresis

Escherichia coli bacterial culture containing plasmids BBa_K759001 (coding region of Ag43 only), was inoculated overnight in LB-chloramphenicol medium in a shaking water bath at 370C. Plasmid mini preps were conducted with a Qiagen plasmid mini prep kit. Single and double restriction digests were carried out accordingly: