Composite
Part:BBa_K1465208:Design
Designed by: iGEM-Team Bielefeld 2014 Group: iGEM14_Bielefeld-CeBiTec (2014-10-02)
Carbonic anhydrase (csoS3) and shell-proteins (csoS14) of the carboxysome of H. neapolitanus
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 166
Illegal AgeI site found at 2277 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Classical [http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#BioBrick Suffix Insertion] of the shell-proteins (BBa_K1465206) into the pSB1C3 backbone of the carbonic anhydrase (BBa_K1465205).
Source
BBa_K1465206: shell proteins csoS4AB and csoS1CAB from Halothiobacillus neapolitanus. BBa_K1465205: carbonic anhydrase csoS3 (can) from Halothiobacillus neapolitanus.