Generator
TnP
Part:BBa_J61023
Designed by: John Anderson Group: Arkin Lab (2006-09-21)
Revision as of 00:00, 22 September 2006 by JCAnderson (Talk | contribs)
Tn5 Transposase [Tnp1-PN] (with PstI and NotI)
High-activity variant of Tn5 transposase-expressing cassette from pRL27. Cassette includes a complete promoter/rbs/orf cassette. The promoter is Ptet, but not derived from r0040. There are PstI and NotI sites in this part, so care must be taken when doing assembly not to use those sites. Part is used in conjunction with transposons derived from J61021 and J61022.
pSB1A2-Bca1014
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 885
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1220
Illegal PstI site found at 885
Illegal NotI site found at 798 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1720
Illegal XhoI site found at 690 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 885
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 885
Illegal NgoMIV site found at 73
Illegal AgeI site found at 1208 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1352
Illegal BsaI.rc site found at 1173
Illegal SapI site found at 309
Illegal SapI.rc site found at 621
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Categories
Parameters
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