Generator
TnP

Part:BBa_J61023

Designed by: John Anderson   Group: Arkin Lab   (2006-09-21)
Revision as of 00:00, 22 September 2006 by JCAnderson (Talk | contribs)

Tn5 Transposase [Tnp1-PN] (with PstI and NotI)

High-activity variant of Tn5 transposase-expressing cassette from pRL27. Cassette includes a complete promoter/rbs/orf cassette. The promoter is Ptet, but not derived from r0040. There are PstI and NotI sites in this part, so care must be taken when doing assembly not to use those sites. Part is used in conjunction with transposons derived from J61021 and J61022.

pSB1A2-Bca1014

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 885
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1220
    Illegal PstI site found at 885
    Illegal NotI site found at 798
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1720
    Illegal XhoI site found at 690
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 885
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 885
    Illegal NgoMIV site found at 73
    Illegal AgeI site found at 1208
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1352
    Illegal BsaI.rc site found at 1173
    Illegal SapI site found at 309
    Illegal SapI.rc site found at 621


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