Composite

Part:BBa_K1378005

Designed by: Meng Liuyi & Zhang Zijian   Group: iGEM14_Peking   (2014-10-03)
Revision as of 05:41, 3 October 2014 by Registry (Talk | contribs)

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INPNC-linker2-MVN-linker1-mRFP

This composite part is fused by INP,MVN and mRFP.It can display MVN protein on the surface of E.coli through INP and emission red fluorecence. We use this part to detect whether MVN is translocated on the out membrane.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 466
    Illegal AgeI site found at 1915
    Illegal AgeI site found at 2027
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1029


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Categories
Parameters
None