Regulatory

Part:BBa_K1402010

Designed by: Chen Xi   Group: iGEM14_NJU-QIBEBT   (2014-09-29)
Revision as of 08:18, 2 October 2014 by ChenXi (Talk | contribs)

Lac Promoter

This part is an inverting regulator sensitive to LacI (BBa_C0010, BBa_C0012, etc.) The T7-lac promoter is a hybrid regulatory region consisting of the T7 promoter of T7 phage next to LacO. The hybrid design allows for strong promotion that can nevertheless be: (1)repressed by LacI. (2)induced by lac or IPTG, showing a high expression level. (3)CAP protein will not influence the expression. It is a strong promoter. You can Only use this part in E.Coli BL21(DE3).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//collections/biofab/promoter
//direction/forward
//dna/nucleotide
Parameters
biology
control