Part:BBa_K1402005

RBS-AtFatA-Terminator

Acyl-acyl carrier protein (ACP) thioesterase catalyzes the hydrolysis of acyl-ACP to fatty acids and ACP this reaction removes the coupling between fatty acid biosynthesis and phospholipid biosyntheis,which is a key to obtain free fatty acids. This thioesterase AtFatA can produce fatty acids with 16~18 carbon chain length. In previous researches, thioesterase gene AtFatA can be expressed in E.coli BL21(DE3) and enhance the synthesis of free fatty acids.This thioesterase can not express itself. The RBS is in front of the AtFatA coding sequence and T7 terminator is after AtFatA. You can design your own promoter for this part, but use this part in E.coli BL21(DE3) only or you’d better delete the T7 terminator. Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 377
  Illegal BglII site found at 552
  Illegal XhoI site found at 935
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 1275