Translational_Unit

Part:BBa_K1465204:Design

Designed by: iGEM-Team Bielefeld 2014   Group: iGEM14_Bielefeld-CeBiTec   (2014-09-22)
Revision as of 00:14, 2 October 2014 by Tore (Talk | contribs) (→‎References)


Shell-associated protein (csoS2) of the carboxysome of Halothiobacillus neapolitanus


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 185
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 343
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 851
    Illegal AgeI site found at 1802
    Illegal AgeI site found at 2483
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 243


Design Notes

Gensynthesis (gBlocks) and PCR-amplification


Source

Gibson-Assembly

References

  • Walter Bonacci, Poh K. Teng, Bruno Afonso, Henrike Niederholtmeyer, Patricia Grob, Pamela A. Silver

and David F. Savage (2011) Modularity of a carbon-fixing protein organelle [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3258634/pdf/pnas.201108557.pdf Proc Natl Acad Sci U S A. 109: 478–483.]