Composite
Ag43 RCF10

Part:BBa_K1352000

Designed by: Ana-Maria Cujba, Martyna Sroka   Group: iGEM14_Aberdeen_Scotland   (2014-10-01)
Revision as of 22:15, 1 October 2014 by Registry (Talk | contribs)

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Ag43 with PstI sites removed under the control of pBad/araC promoter

BBa_K1352000 is the modularized version of BBa_K759001, which is compatible with iGEM Assemly Standard RCF10.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1945
    Illegal NgoMIV site found at 3169
    Illegal NgoMIV site found at 3679
    Illegal NgoMIV site found at 3700
    Illegal AgeI site found at 979
    Illegal AgeI site found at 2695
    Illegal AgeI site found at 3439
    Illegal AgeI site found at 3853
    Illegal AgeI site found at 4095
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


[edit]
Categories
Parameters
None