Device

Part:BBa_K1329001

Designed by: Daniela Geist   Group: iGEM14_Marburg   (2014-10-01)
Revision as of 20:20, 1 October 2014 by Registry (Talk | contribs)

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Hag-KpnI

Hag-KpnI consists of the Bacillus subtilis flagellin gene which as designed with a KpnI site to integrate different domains, tags or modules with a Gibson assembly after linearizing the vector with KpnI. Using a shuttle vector for gam-positive bacteria it is possible to integrate the modified flagellin construct into the Bacillus genome considering a special heat shock protocoland specific flanks besides the used gene locus. By engineering new modules into the conserved structure of flagellin Bacillus is able to form flagella build up from those modified flagellins.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 588


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Parameters
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