Part:BBa_K1329000
StrepDARPidin
StrepDARPidin is a fusion protein combining the monomeric streptavidin from Streptomyces avidinii and the DARPin (Designed Ankrin Repeat Protein) EC1 for targeting a tumor surface marker. Streptavidin monomers build up tetrameric constructs and have high and specific affinity to biotin or Strep-Tags. The DARPin EC1 was designed for an specific binding of EpCAM which is a surface marker of tumor cells derived from epithelial tissue. Our idea was to create a fusion protein which can be used for targeting appropiate cells with increased affinity by creating induced proximity using tetrameric structures. Additionally these can be used for linking biotinylated or Strep-tagged constructs at the same time.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 598
- 1000COMPATIBLE WITH RFC[1000]
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