Composite

Part:BBa_K1453000:Design

Designed by: Renhe Luo   Group: iGEM14_SJTU-BioX-Shanghai   (2014-10-01)
Revision as of 15:09, 1 October 2014 by Registry (Talk | contribs)

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ssDsbA-mRFP-Lgt-TAL USB-His Tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 612
    Illegal AgeI site found at 724
    Illegal AgeI site found at 1537
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1158


Design Notes

When digest with BsmBI, this part can create two sticky-ends, one can match with the first TAL-Protein DiRepeat (Bba_K747000 to Bba_K747015) at 5` of the sequence, the other can match with the sixth TAL-Protein DiRepeat (Bba_K747080 to Bba_K747095) at 3`. With the rest of TAL-Protein DiRepeats (Bba_K747016 to Bba_K747079), user can synthesize a 14 nucleotide Transactivator-like (TAL) protein to suit for their own connector and design their own polymerization. (Golden Gate Cloning) More details about membrane anchor, please find http://2012.igem.org/Team:SJTU-BioX-Shanghai. More details about TAL and Golden Gate Cloning, please find http://2012.igem.org/Team:Freiburg.


Source

BBa_K771000 BBa_E1010

References