Part:BBa_K1470001
puromycin N-acetyl-transferase
Puromycin is an antibodic derived from Streptomyces alboniger. The substance affects the cell both in protein translation and mitochondrial protein import. Due to this, it's toxic to many organisms such as bacteria, Trypanosoma and humans.
A small part of its structure resembles to aminoacetylated tRNA and is recognized by ribosomes. the nascent chain containing puromycin leads to a translational stop.
Streptomyces alboniger protects itself from puromycin with a acetyl transferase. It uses acetyl-CoA to bind the the acetyl group with the amino group. In this state puromycin cannot be recognized by the ribosom anymore.
This enzyme is often used for selecting stable eucaryotic cell lines. Because of the blocked protein biosynthesis nearly over 99 % of uninfected cells will be dead within two days.
References:
PUROMYCIN INHIBITION OF PROTEIN SYNTHESIS., DARKEN MA.,Pharmacol Rev. 1964 Sep;16:223-43., PMID:14211122
NEUBERT, D. AND LEHNINGER, A. L.: The effect of oligomycin, gramicidin, and other antibiotics on reversal of mitochondrial swelling by adenosine triphosphate. Biochim. biophys. Acta 62: 556-565, 1962., http://dx.doi.org/10.1016/0006-3002(62)90238-X, http://www.sciencedirect.com/science/article/pii/000630026290238X
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 481
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