Not Released
Sample In stock
Experience: Works
Not Used
Get This Part
Translational_Unit

Part:BBa_K1429002

Designed by: Christal Gordon   Group: iGEM14_Genspace   (2014-09-29)
Revision as of 01:13, 1 October 2014 by Cgordon (Talk | contribs)

Cyan Fluorescent Protein (CFP) "Cindy Lou" coding region, intellectual property-free

IP free cyan fluorescent protein. We have a ribosome binding site (RBS).

Background

Making sure the inserts are here from the colony PCR results - are the GFP and RFP inserts present?


Digest each plasmid with EcoRI/PstI. Incubate 15' at 37C.


Run entire digest on 0.8% gel.


Results

These were slightly confounding. What I *think* happened is that the buffer wasn't optimal for one of the enzymes, so the digest wasn't run to completion. Why do I think this?

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 74
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2


[edit]
Categories
Parameters
None