Coding
MSA

Part:BBa_K1392932:Design

Designed by: Burak Kızıl   Group: iGEM14_METU_Turkey   (2014-09-29)
Revision as of 01:53, 30 September 2014 by Maercyl (Talk | contribs) (→‎References)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


4-hydroxy-2-ketovalerate aldolase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 813
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In order to take 4-hydroxy-2-ketovalerate aldolase from Escherichia coli BL21(DE3)'s complete genome a primer has designed and to get 1014 bp, the part has connected to CHL backbone.


Source

Escherichia coli BL21 (DE3)

References

http://www.ncbi.nlm.nih.gov/pubmed?LinkName=nuccore_pubmed&from_uid=387825439

http://www.ncbi.nlm.nih.gov/nuccore/387825439?from=343403&to=344416&sat=4&sat_key=99161499&report=gbwithparts