Conjugation
OriTRP4

Part:BBa_K1439000:Design

Designed by: Wenqi Li   Group: iGEM14_OUC-China   (2014-09-28)
Revision as of 01:48, 30 September 2014 by Tifalee (Talk | contribs) (→‎Design Notes)

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Origin of transfer for the RP4-plasmid nic region.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 20
    Illegal NgoMIV site found at 30
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

OriTRP4 region contains some promoters, we put it in the back of terminator.In order to reduce the impact on protein expression.

Source

This part comes form the RP4 plasmid, from 51098bp to 51448bp.

References