Part:BBa_F2620

A work in progress...'

Description
A transcription factor [LuxR] that is active in the presence of cell-cell signaling molecule 3OC6HSL is controlled by an operator [TetR]. Device input is 3OC6HSL. Device output is PoPS produced at a LuxR-regulated operator.

Usage
Full PoPS output at high 3OC6HSL levels and high plasmid copy [e.g., Part:pSB1A2] results in a reduced cell growth rate (see Load section). If used in a cell containing TetR then a second input signal such as [http://openwetware.org/wiki/ATc aTc] can be used to produce a logical AND function.

Characteristics Input Swing: ## nM 3OC6HSL, exogenous Output Swing: #-# PoPS Switch Point: 2 nM 3OC6HSL, exogenous L/H Latency: # minutes

Key Subparts BBa_R0040: TetR-regulated operator BBa_C0062: luxR ORF BBa_R0062: LuxR-regulated operator

Transfer Function	Specificity
Latency	Stability

Demand Transcription Demand: ## NTP per second; ## polymerases sequestered Translation Demand: # charged tRNA per second; # ribosomes sequestered

Stability Genetic Stability: ##/## replication events (Lo Input/High Input) Performance Stability: ##/## replication events (Lo Input/High Input)

Compatibility
Device has been shown to work in BBa_V1002, MG1655 and BBa_V1001
Device has been shown to work on pSB3K3 and pSB3K3
Device has been shown to work with BBa_E0430,BBa_E0434,BBa_E0240
Crosstalk with devices using 3OC₆HSL or add in the other molecule names here. Crosstalk with systems containing TetR (BBa_C0040)

3OC₆HSL -> PoPS Receiver

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1004
  

Device Characteristics

control	R0040
device_type	Receiver
family	-NA-
latency	Seconds
proteins	LuxR, TetR
signalling_molecule	3OC6HSL
switch_point	2nM

Get the device [http://openwetware.org/wiki/Image:F2620.de2-ania.ppt#filelinks datasheet]

Specificity

[http://openwetware.org/images/9/93/Transfer.jpg Specificity preliminary data]

Stability

[http://openwetware.org/images/b/b9/StabilitySlideCropped.jpg Stability preliminary data]