Reporter

Part:BBa_K1555002:Design

Designed by: Xu Xuan   Group: iGEM14_NJAU_China   (2014-09-28)
Revision as of 20:15, 28 September 2014 by Xuan (Talk | contribs) (→‎References)

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The cueo promoter with a gfp reporter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 776


Design Notes

The sequence we chosed in cueo includes a putative position which may be directly bound by CueR.



Source

E.coli's genome

References

Outten, F. W., Outten, C. E., Hale, J., & O'Halloran, T. V. (2000). Transcriptional Activation of an Escherichia coli Copper Efflux Regulon by the Chromosomal MerR Homologue, CueR. Journal of Biological Chemistry,275(40), 31024-31029.