Reporter
Part:BBa_K1555000
Designed by: Xu Xuan Group: iGEM14_NJAU_China (2014-09-28)
The copa promoter wth a gfp reporter
This part is our testing part.The copa is one of the candidates of our copper sensor.For comparing with other two sequences,marRAB and cueo,we linked a reporter(BBa_E0840) follow the promoter of copa.With the concentration of copper rises, the expression of downstream gfp coding gene increases.We designed two experiments to test those candidates' sensitivity and specificity individually by measure fluorescence intensity.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 914
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Categories
Parameters
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