Composite

Part:BBa_K1433005

Designed by: Hanqing Liu   Group: iGEM14_ZJU-China   (2014-09-28)
Revision as of 16:28, 28 September 2014 by Registry (Talk | contribs)

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RBS-gamma protein-beta protein-exonuclease

This part is constructed for improve BBa_K1223012, pKD46 functional unit. Lambda red & pKD46 brief introduction. 

 BBa_K1223012 is not conform to any RFC cause there are three restrict enzyme sites of EcoRI and PstI. As for improve the compatibility of this part and make these useful modules be used in future assemblies, we remove these restrict enzyme sites by synonymous mutations


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1422
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None