Composite

Part:BBa_K1433003

Designed by: Hanqing Liu   Group: iGEM14_ZJU-China   (2014-09-28)
Revision as of 16:22, 28 September 2014 by Rubisco (Talk | contribs)

RBS-gp47-tag-terminator

Bxb1 gp47 is an excisionase and Bxb1 gp35 is a serine integrase in Mycobacterium phage Bxb1. This intergrase along can typically catalyzes site-specific recombination and mediate DNA inversion from an BP to LR state. Heterologous expression of Bxb1 integrase gp35 and excisionase gp47 could mediate DNA restore from an LR to BP state. 

This part is composed of 4 elements.

1. Promoter: BBa_J23110, a middle-ground Bacterial constitutive promoter. 2. gp47: An excisionase in Mycobacterium phage Bxb1. 3. tag: A distinct proteolysis tag on gp47 peptides. 4. terminator: BBa_B0015, a strong double terminator. This part has component encodes a distinct proteolysis tag on gp47 peptides, for fine control over gp47 synthesis and degradation.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 29
    Illegal AgeI site found at 797
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None