Intermediate

Part:BBa_S03595:Experience

Designed by: Karmella Haynes   Group: iGEM06_Davidson   (2006-09-14)
Revision as of 22:26, 14 September 2006 by Kahaynes (Talk | contribs) (→‎Applications of BBa_S03595)


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Applications of BBa_S03595

This part was constructed to test the capacity of the Double Forward Terminator [BBa_B0015] to block read-through transcription.

Part [BBa_S03562], which contains TetR with a ribosomal binding site, is sufficient to convey tetracycline resistance in the absence of a promoter. We suspected that TetR might be expressed via read-through transcription from the carrier vector.

Consistent with this observation, the following parts are also expressed in the absence of an upstream promoter:

  • Insert list here

Once the Double Forward Terminator [BBa_B0015] is placed upstream of RBS-TetR, the cells are no longer tetracycline resistant. We conclude that the Double Forward Terminator is sufficient to block read-through transcription, allowing control over the expression of the downstream coding region.

Construction of an "insulator vector" where the double terminator is placed before the BioBrick prefix and after the BioBrick suffix (in the reverse orientation) is currently under way.

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