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Coding

Part:BBa_K1554001

Designed by: Valencia_UPV team members   Group: iGEM14_Valencia_UPV   (2014-09-26)
Revision as of 15:46, 28 September 2014 by Lestelles (Talk | contribs)

HarFAR

Delta 11 desaturase from Amyelois transitella. Codon optimization was perfomed for Nicotiana benthamiana.


Usage and Biology

The AtrDelta11 protein is a delta 11 desaturase coming from Amyelois transitella which introduces a desaturation in fatty acids at the 11th bond.

acyl-CoA + reduced acceptor + O2 = Delta11-acyl-CoA + acceptor + 2 H2O

Our team used it in with Part:BBa_K1554001 to produce the insect pheromone Z11-16:OH in Nicotiana benthamiana using palmitate:CoA as precursor. It was also used with Part:BBa_K1554003 to transform Z11-16:OH into antoher insect pheromone, Z11-16:OAc.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1104
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 929


[edit]
Categories
//cds
//cds/biosynthesis
//cds/enzyme
//chassis/eukaryote/nbenthamiana
//function/biosynthesis
Parameters
kegghttp://www.genome.jp/dbget-bin/www_bget?enzyme+1.14.19.5
uniprothttp://www.uniprot.org/uniprot/S4WAY4