Composite

Part:BBa_K1363201

Designed by: CHANGXU WANG   Group: iGEM14_USTC-China   (2014-09-28)
Revision as of 11:24, 28 September 2014 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


Anti-LPS factor(LALF) regulated by IPTG

This part is a cell-kill system. Firstly,constitutive promoter could express enough Lacl that is useful for downstream. Secondly, the reporter in the middle of our part wouldn't show signal nor the cell be killed by the part behind unless IPTG or lactose added into the culture medium. No IPTG or lactose: GFP don't express ,G- won't be killed. IPTG or lactose exsisting : GFPs express ,G- will be dead.

G-:Gram negative bacteria

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1208
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2085


[edit]
Categories
//biosafety
Parameters
None