Device

Part:BBa_K1491036

Designed by: Danielle Peters   Group: iGEM14_Carnegie_Mellon   (2014-09-26)
Revision as of 18:14, 26 September 2014 by Registry (Talk | contribs)

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Constitutive Dead ER Intein T7 RNAP with YFPH

T7 RNA polymerase that contains the dead version estrogen receptor intein with a constitutive promoter. YFP is added to the end of the plasmid so that the amount of T7 RNA polymerase that is produced can be quantified through fluorescence analysis of YFP. Used as a control.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2525
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2950
    Illegal AgeI site found at 2914
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2425


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Categories
Parameters
None