DNA

Part:BBa_K1560000

Designed by: Christopher Lee   Group: iGEM14_UCSC   (2014-09-26)
Revision as of 18:06, 26 September 2014 by Registry (Talk | contribs)

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-- No description --

This construct can be used to knockout the acyl-CoA dehydrogenase 2 gene in Haloferax Volcanii through Thorston Allers' pop-in pop-out method(Halo Handbook). Thus the sequence is composed of 400 base pairs of homologous upstream genomic DNA, the first 50 base pairs of Acd2 in Haloferax Volcanii, 99 base pairs of nonsense DNA, the final 50 base pairs of Acd2, followed by 400 base pairs of homologous downstream genomic DNA. The product of successful plasmid integration and treatment with 5-FOA results in the Acd2 gene replaced by the 99 base pairs of nonsense DNA.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 737
    Illegal NgoMIV site found at 947
    Illegal NgoMIV site found at 1496
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 111
    Illegal BsaI site found at 249
    Illegal BsaI site found at 1019
    Illegal BsaI site found at 1926
    Illegal BsaI.rc site found at 1685


[edit]
Categories
Parameters
None