Regulatory

Part:BBa_J100181:Experience

Designed by: Paige Farrar   Group: Campbell M Lab   (2014-09-11)
Revision as of 19:40, 25 September 2014 by Hewebster (Talk | contribs) (→‎Applications of BBa_J100181)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_J100181

SoxSwithpositivecontrol.png



SoxSgraph.png


After further investigation, the addition of the hydrogen peroxide to the samples including the SoxS promoter and the negative control appears to have killed all the cells. The concentration of hydrogen peroxide, therefore was too high in order to simply increase oxidative stress, and ultimately produced ineffective results. The data is inclusive whether the soxR protein successfully repressed the SoxS promoter, or the cell was simply incapacitated by the addition of the hydrogen peroxide. The graphs of the data are above. The first graph includes the positive control, but the second graph excludes this sample in order to make the ratios of the experimental and negative control samples more visible.

User Reviews

UNIQ268f8542dc493097-partinfo-00000000-QINU UNIQ268f8542dc493097-partinfo-00000001-QINU