Regulatory

Part:BBa_K1513000

Designed by: Hongwei Duan   Group: iGEM14_BUCT-China   (2014-09-18)
Revision as of 16:00, 24 September 2014 by Lllazy (Talk | contribs)

A quorum sensing system which can produce auto-inducer (AHL) itself.

Our part is constituted by Plac、luxR、Pluxr、luxr. LuxI can result in the production of AHL—a kind of auto-inducer which can bind to the protein LuxR and induce the high expression of downstream genes of Pluxr，therefore，this system can drive a mass of bacteria highly express a particular gene. What’s more, AHL can be used by all bacteria in certain range.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1710
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 987


[edit]
Categories
Parameters
None