Regulatory

Part:BBa_K1467101

Designed by: Cara Deal   Group: iGEM14_NRP-UEA-Norwich   (2014-09-24)
Revision as of 13:52, 24 September 2014 by Registry (Talk | contribs)

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GoldenGate compatible 35s Promoter

35S is a plant specific promoter obtained from Cauliflower Mosaic Virus. The part is intended for use as a constitutive promoter for gene expression experiments.

The part is compatible with GoldenGate MoClo Assembly Standard (free from BsaI and BpiI recognition sequences).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 392
    Illegal BglII site found at 1138
    Illegal XhoI site found at 1342
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/eukaryote/nbenthamiana
//promoter
//regulation/constitutive
Parameters
None