Coding

Part:BBa_K1333203

Designed by: Yanwen Xie   Group: iGEM14_SYSU-China   (2014-09-23)
Revision as of 04:26, 24 September 2014 by Registry (Talk | contribs)

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lambda cI-LGF2(D76A&D78A)

Fuse lambda cI with LGF2 mutated from Asp to Ala at sites 76 and 78 (the Glu which locates after three continuous Ala behind lambda repressor is at site 58). As one of the components in Bacterial Two-hybrid System, it causes little interaction with GAL11P compared to the normalized WT.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 712
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 767
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/ecoli
//proteindomain/activation
//proteindomain/binding
Parameters
familywith BBa_K1333200, BBa_K1333201, BBa_K1333202
functionhardly interact with Gal11P
proteinfusion protein of lambda cI and LGF2