Coding
Part:BBa_K1333200
Designed by: Yanwen Xie Group: iGEM14_SYSU-China (2014-09-23)
lambda cI-LGF2(E75A)
Fuse lambda cI with LGF2 mutated from Glu to Ala at site 75 (the Glu which locates after three continuous Ala behind lambda repressor is at site 58). As one of the components in Bacterial Two-hybrid System, it causes little interaction with GAL11P compared to the normalized WT.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 712
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 767
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//chassis/prokaryote/ecoli
//proteindomain/activation
//proteindomain/binding
//proteindomain/activation
//proteindomain/binding
family | with BBa_K1333201, BBa_K1333202, BBa_K1333203 |
function | hardly interact with Gal11P |
protein | fusion protein of lambda cI and LGF2 |