Composite

Part:BBa_K1520509

Designed by: yue wu   Group: iGEM14_Nanjing-China   (2014-09-23)
Revision as of 02:32, 24 September 2014 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


PgolTS-golS-PgolB-rbs-tetR-Ter-PtetO-rbs-rfp-Ter-Plac-rbs-tetR-Ter-Pcons2-rbs-lacI-Ter

PgolTS-golS-PgolB-rbs-tetR-Ter-PtetO-rbs-rfp-Plac-rbs-tetR-Ter-Pcons2-rbs-lacI-Ter It is a test of our suicide circuit for gold ions.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3611
    Illegal NheI site found at 3634
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 4812
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 548
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/ecoli
//direction/forward
//promoter
//regulation/negative
//rnap/prokaryote/ecoli/sigma70
Parameters
biology
controlaTc, tetracyline
directionForward
functionRep
ligandstetracycline
n/aconstitutive promoter family member
negative_regulators1
o_h
o_l
positive_regulators
proteinTetR
swissproP04483
tagLVA