Coding

Part:BBa_K1365000:Design

Designed by: Sandra Mous   Group: iGEM14_Groningen   (2014-09-23)
Revision as of 08:40, 23 September 2014 by S.Mous (Talk | contribs) (→‎Design Notes)


NisA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Used primers:
forward - 5'-GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAGTACAAAAGATTTTAA-3'
reverse - 5'-GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTATTTGCTTACGTGAA-3'
to PCR NisA from the genome and add the BioBrick prefix and suffix.

Source

Obtained by PCR on the genome of L. lactis NZ9700, a nisin producing strain.

References