Reporter
Part:BBa_K1399010
Designed by: Anna Stikane Group: iGEM14_Edinburgh (2014-09-19)
Revision as of 16:05, 19 September 2014 by AStikane (Talk | contribs) (Created page with " == Plac-RFP(AAV) == Lactose/IPTG inducible promoter with RFP reporter tagged with AAV-ssrA degradation tag followed by terminator. The tagged RFP is actively degraded within ce...")
Plac-RFP(AAV)
Lactose/IPTG inducible promoter with RFP reporter tagged with AAV-ssrA degradation tag followed by terminator. The tagged RFP is actively degraded within cell, thus it provides better temporal resolution of red fluorescence and promoter activity. Part was assembled using BrickClip assembly (BBF RFC 104) using other biobrick parts as templates. BrickClip assembly is a special case of more general Paperclip assembly method.[1]
[1] Trubitsyna, M., Michlewski, G., Cai, Y., Elfick, A. & French, C. E. PaperClip: rapid multi-part DNA assembly from existing libraries. Nucleic Acids Res. 1–8 (2014). doi:10.1093/nar/gku829
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Categories
Parameters
//cds/reporter/rfp
//classic/generator/prct
//classic/reporter/pret
//function/reporter/fluorescence
//classic/generator/prct
//classic/reporter/pret
//function/reporter/fluorescence
| emission | RFP |
| excitation | |
| tag | SsrA-AAV degradation tag |