Coding

Part:BBa_K1351000

Designed by: Mona Dotzler   Group: iGEM14_LMU-Munich   (2014-09-15)
Revision as of 05:11, 18 September 2014 by Mdotzler (Talk | contribs)

beta-Neurexin-derived peptide binding to S. aureus adhesin SdrC

Short peptide of 11 amino acids corresponding to the N-terminus of the human protein neurexin beta-1. It includes the binding site (HHIHHFH) for the Staphylococcus aureus adhesin SdrC. Displayed on the surface of Bacillus subtilis, it should enable B. subtilis to bind specifically to S. aureus.

This part was generated in RFC25 standard with the following prefix and suffix:
ENX_SD_Ngo_fwd: 5' GAATTC-GCGGCCGC-T-TCTAGA-GT-AAGGAGG-A-GCCGGC-ATG 3'
PNS_Age_rev: 5' CTGCAG-CGGCCG C-T-A CTAGT-ATTA-ACCGGT 3'
Restriction sites in bold, Shine-Dalgarno sequence and stop codons underlined.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds
//chassis/prokaryote/bsubtilis
Parameters
None