Part:BBa_K1412006

RBS (0.01) CheZ double terminators -- derivative of BBa_K1412005

This part consists of a cheZ coding region which is responsible for the dephosphorylation of CheY protein in bacteria flagella movement. This part does not pose any biological threat. With a promoter of your interest, this device rescues the mobility of cheZ-/- cells. It has been reported that cheZ-/- strain has a higher frequency of direction change and thus a narrower range of mobility. This part also has a RBS coding region whose efficiency is 0.3.

Usage

1.We performed a swarming assay to characterize the motility of CheZ-deficient strain CL1 and cells transformed with CheZ-expressing plasmid BBa_K1412001 .

Our results clearly show that CheZ-deficient mutants show smaller diffusion, while they are rescued by our BBa_K1412001 plasmid.

2.We also performed a swarming assay to characterize characterize the strength of RBS with CL1 cells. Three strains of CL1 are transformed with CheZ-expressing plasmid of different RBS efficiency (BBa_B0034 :1.0 ; BBa_B0032 :0.3 ; BBa_B0033 :0.01 ).

Changing different RBS in the same circuit, we can characterize the efficiency of RBS, since the expression strength of CheZ is positively associated with the efficiency of RBS.

Experimental data

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Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Reference

[1] [http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0011915#pone-0011915-g006 Paungfoo-Lonhienne C et al. (2010) Turning the table: plants consume microbes as a source of nutrients. PLoS One 5(7): e11915 ].

[2] [http://pubs.acs.org/doi/abs/10.1021/bi00561a015 Chelsky D and Dahlquist FW (1980) Chemotaxis in Escherichia coli: association of protein components. Biochemistry 19: 4633–4639 ].