Part:BBa_K1412829

Characterize efficiency of RBS with chemotaxis

BBa_K1412829: Plac-RBS(0.01)-CheZ-TT

This part consists of a CheZ gene which can express CheZ protein deciding E.coli whether tumble or swim straight.In this light, we can characterize the RBS and promoter efficiency by just change different promoters or ribosome binding sites.Then we can characterize the efficiency of RBS and promoter via measuring the migration distance positively associated with the expression strength of CheZ.

Usage

When we want to characterize the efficiency of RBS, we usually link the RBS between promoter and GFP, then characterize the RBS by just measure the fluorescence intensity of GFP. In our part, you need just link RBS after a Plac promoter and before a CheZ gene, ending with a TT terminator. Then transfer this gene circuit into E.coli (CheZ knock out), and coat plates, culuture for hours to measure the migration diameter of E.coli.

Relevant parts

BBa_K1412005

BBa_K1412006

BBa_K1412007

BBa_K1412000

Notes

Source 3H:13-P3-3H BBa_R0010 2L:14-P2-2L BBa_B0033 18G:14-P1-18G BBa_K629003 4F:13-P3-4F BBa_B0015

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]