Coding
Part:BBa_K1333201
Designed by: Yanwen Xie Group: iGEM14_SYSU-China (2014-09-09)
lambda cI-LGF2(D78A)
Fuse lambda cI with LGF2 mutated from Asp to Ala at site 78. As one of the components in Bacterial Two-hybrid System, it causes a less interaction with GAL11P compared to the nomalized WT.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 712
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 767
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Functional Parameters
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References:
name of the paper
| family | with BBa_K1333200, BBa_K1333202, BBa_K1333203 |
| function | hardly interact with Gal11P |
| protein | fusion protein of lambda cI and LGF2 |
[edit]
Categories
Parameters
//chassis/prokaryote/ecoli
//proteindomain/activation
//proteindomain/binding
//proteindomain/activation
//proteindomain/binding
| family | with BBa_K1333200, BBa_K1333202, BBa_K1333203 |
| function | hardly interact with Gal11P |
| protein | fusion protein of lambda cI and LGF2 |