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Coding

Part:BBa_K1333201

Designed by: Yanwen Xie   Group: iGEM14_SYSU-China   (2014-09-09)
Revision as of 03:27, 10 September 2014 by Registry (Talk | contribs)

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lambda cI-LGF2(D78A)

Fuse lambda cI with LGF2 mutated from Asp to Ala at site 78. As one of the components in Bacterial Two-hybrid System, it causes a less interaction with GAL11P compared to the nomalized WT.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 712
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 767
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/ecoli
//proteindomain/activation
//proteindomain/binding
Parameters
familywith BBa_K1333200, BBa_K1333202, BBa_K1333203
functionhardly interact with Gal11P
proteinfusion protein of lambda cI and LGF2