Measurement

Part:BBa_K1412716

Designed by: Jielin Chen, An Chen   Group: iGEM14_XMU-China   (2014-09-02)
Revision as of 08:16, 2 September 2014 by AlexLeBron (Talk | contribs)

GFP generator with J23101

This part consists of Anderson promoter J23101 which is from the Anderson collection without RFP in backbone vector pSB1C3 to easily fuse the promoter with other reporters e.g. the lux operon or the lacZ reporter gene. This part was also evaluated in the publication The Bacillus BioBrick Box: generation and evaluation of essential genetic building blocks for standardized work with Bacillus subtilis by Radeck et al..

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 706


[edit]
Categories
Parameters
None