Part:BBa_K1486009

CxpR & Split IFP1.4 [Nterm + Nterm]

This part has two fused proteins downstream of an arabinose-inducible promoter: CpxR-IFP[1] and CpxR-IFP[2].

This construct was made in all orientations:

- CpxR-IFP[1] + CpxR-IFP[2] (BBa_K1286008)

- IFP[1]-CpxR + CpxR-IFP[2] (BBa_K1486010)

- CpxR-IFP[1] + IFP[2]-CpxR (BBa_K1486011)

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 1481
  Illegal PstI site found at 2714
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1205
  Illegal PstI site found at 1481
  Illegal PstI site found at 2714
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 2925
  Illegal BamHI site found at 1144
  Illegal XhoI site found at 1718
  Illegal XhoI site found at 3085
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 1481
  Illegal PstI site found at 2714
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 1481
  Illegal PstI site found at 2714
  Illegal AgeI site found at 979
  Illegal AgeI site found at 2129
  Illegal AgeI site found at 3496
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 961